Purification of resveratrol from vine stems.

Autor: Aaviksaar, Aavo, Haga, Mati, Püssa, Tõnu, Roasto, Mati, Tsoupras, George
Předmět:
Zdroj: Proceedings of the Estonian Academy of Sciences, Chemistry; Dec2003, Vol. 52 Issue 4, p155-164, 10p
Abstrakt: A modified method for preparative purification of trans-resveratrol (trans-3,4 ',5-tri-hydroxystilbene) from vine stems is presented. It includes two new procedures added to the known scheme of resveratrol separation from ligneous organs of vine by ethanol--water extraction: (1) treatment of ethanolic solution of vine stem stilbenoid extract with diethyl ether for precipitation of a dark brown matter from the extract; (2) preparative column chromatography on polyamide carrier for separation of resveratrol from the crude ethanolic extract. As a result, 93.8% resveratrol concentrate with 0.7% of its dimer viniferin impurity was obtained. Resveratrol and viniferin contents in the stems of various frost-hardy hybride cultivars of Vitis vinifera grown open air in Estonia ('Hasaine Sladki', 'Jubilei Novgoroda', Vitis vinifera cv., 'ES 12-7-98', 'Marechal Joffre', 'Zilga') were determined for the plant material collected in July and in October. Lignified stems collected in October turned out to be an excellent source for producing vine stem stilbenoid (resveratrol and viniferin) concentrates for use as healthy ingredients in functional food for preventing heart diseases and atherosclerosis. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index