| Autor: |
Croissant, Jonas G., Mauriello Jimenez, Chiara, Wong Chi Man, Michel, Raehm, Laurence, Durand, Jean-Olivier, Picard, Sébastien, Klausen, Maxime, Clermont, Guillaume, Genin, Emilie, Blanchard-Desce, Mireille, Aggad, Dina, Garcia, Marcel, Gary-Bobo, Magali, Maynadier, Marie, Mongin, Olivier, Cattoën, Xavier |
| Zdroj: |
Journal of Materials Chemistry B; 9/7/2016, Vol. 4 Issue 33, p5567-5574, 8p |
| Abstrakt: |
The synthesis of ethenylene-based periodic mesoporous organosilica nanoparticles for two-photon imaging and photodynamic therapy of breast cancer cells is described. A dedicated two-photon absorbing fluorophore possessing four triethoxysilyl groups and having large two-photon absorption in the near IR region, and azidopropyltriethoxysilane were incorporated into the structure. The mesoporous nanoparticles of 100 nm diameter were further functionalized by means of click chemistry with a propargylated fluorescent bromo-quinoline photosensitizer able to generate singlet oxygen. The photophysical properties and two-photon absorption properties of the nanoparticles were investigated evidencing complementary contribution of the two dyes. Both dyes contribute to the two-photon absorption response of the mesoporous nanoparticles while efficient FRET from the two-photon fluorophore to the quinoline sensitizer is observed. The dual-functionalized nanoparticles were incubated with MCF-7 breast cancer cells. Two-photon confocal imaging demonstrated the endocytosis of the nanoparticles within cancer cells. Moreover, brief two-photon irradiation (3 scans of 1.57 s) at 760 nm at high laser power (3 W) was shown to induce 40% of cancer cell death demonstrating the potential of the dual-functionalized mesoporous organosilica nanoparticles for two-photon photodynamic therapy. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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