| Abstrakt: |
The aim of the research was to explore the optimum condition of ultrasound combined with semi-bionic extraction of Mollugo pentaphylla flavonoids and investigate the antioxidant activity of flavonoids. Ultrasound combined with semi-bionic method was used to extract Mollugo pentaphylla flavonoids. Orthogonal test was applied to find out the optimum conditions for the following factors: temperature, ethanol concentration, extraction time and solid-liquid ratio. 2,2-Diphenyl-1-picrylhydrazyl (DPPH) method was used to investigate the antioxidant activity. Orthogonal test results suggested that the optimum conditions for the extraction of Mollugo pentaphylla flavonoids by ultrasound combined with semi-bionic method were: temperature 60°C, ethanol concentration of 80%, extraction time of 20 min, and solid-liquid ratio of 1:16. Antioxidant activity experiment results demonstrated that the scavenging rate of DPPH by 12.6 μg/ml Mollugo pentaphylla flavonoids was 94.1%, and the scavenging capacity was increasing as flavonoids concentration increased within tested range. As a convenient and economical method, the technology of ultrasound combined with semi-bionic could be applied to the extraction of Mollugo pentaphylla flavonoids. The antioxidant activity study demonstrated Mollugo pentaphylla flavonoids with antioxidant capacity, and the antioxidant activity increased as the flavonoids concentration increased. [ABSTRACT FROM AUTHOR] |
