| Autor: |
Kalai‐Grami, Leila, Karkouch, Ines, Naili, Omar, Slimene, Imen Ben, Elkahoui, Salem, Zekri, Roudaina Ben, Touati, Ines, Mnari‐Hattab, Monia, Hajlaoui, Mohamed Rabeh, Limam, Ferid |
| Předmět: |
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| Zdroj: |
Journal of Basic Microbiology; Aug2016, Vol. 56 Issue 8, p864-871, 8p |
| Abstrakt: |
A lipopeptide-producing endophytic Bacillus methyltrophicus TEB1 strain exhibited potent antifungal activity against Phoma tracheiphila. Lipopeptide production started at the early growth phase plateaued after 36 h of culture where it reduced the mycelium growth by 80%. The crude lipopeptide extract harvested at the stationary phase efficiently inhibited the growth of P. tracheiphila mycelium and MIC values displaying 50 and 90% inhibition of conidia germination were around 47.5 and 100 μg ml−1, respectively. Increasing lipopeptide extract till 3 mg ml−1 induced 10% swelling and 3% crumbling of P. tracheiphila conidia whereas 5 mg ml−1 induced 40% swelling and 20% crumbling. Mass spectrometry analysis of the lipopeptide extract indicated that surfactin production took place from 12 to 20 h, iturin A from 16 to 72 h, and fengycin from 12 to 72 h and that the main active compound against P. tracheiphila was identified as C15 iturin A lipopeptide. Iturin A appeared as a potential biological control agent able to substitute the currently used chemical pesticides in agriculture. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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