Re-engineering a NiFe hydrogenase to increase the H2 production bias while maintaining native levels of O2 tolerance.

Autor: Flanagan, Lindsey A., Wright, John J., Roessler, Maxie M., Moir, James W., Parkin, Alison
Předmět:
Zdroj: Chemical Communications; 7/25/2016, Vol. 52 Issue 58, p9133-9136, 4p
Abstrakt: Naturally occurring oxygen tolerant NiFe membrane bound hydrogenases have a conserved catalytic bias towards hydrogen oxidation which limits their technological value. We present an Escherichia coli Hyd-1 amino acid exchange that apparently causes the catalytic rate of H2 production to double but does not impact the O2 tolerance. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index