Multimodal In Vivo Imaging of Tumorigenesis and Response to Chemotherapy in a Transgenic Mouse Model of Mammary Cancer.

Autor:	Alberini, Jean-Louis, Boisgard, Raphaël, Guillermet, Stéphanie, Siquier, Karine, Jego, Benoît, Thézé, Benoît, Urien, Saik, Rezaï, Keyvan, Menet, Emmanuelle, Maroy, Renaud, Dollé, Frédéric, Kühnast, Bertrand, Tavitian, Bertrand, Boisgard, Raphaël, Guillermet, Stéphanie, Jego, Benoît, Thézé, Benoît, Rezaï, Keyvan, Dollé, Frédéric, Kühnast, Bertrand
Předmět:	NEOPLASTIC cell transformation LABORATORY mice CANCER chemotherapy DRUG therapy ANTINEOPLASTIC agents CARCINOGENESIS ANIMAL experimentation BIOLOGICAL models CELL physiology COMPUTED tomography DEOXY sugars DIAGNOSTIC imaging LIGHT MICE RADIOPHARMACEUTICALS TECHNETIUM TIME TUMOR markers SINGLE-photon emission computed tomography DISEASE progression PHARMACODYNAMICS
Zdroj:	Molecular Imaging & Biology; Aug2016, Vol. 18 Issue 4, p617-626, 10p
Abstrakt:	Purpose: Transgenic mice expressing the polyoma middle T oncoprotein (PyMT) in the mammary epithelium were explored by multimodal imaging to monitor longitudinally spontaneous tumor growth and response to chemotherapy.Procedures: Positron emission tomography (PET) with 2-deoxy-2-[(18)F]fluoro-D-glucose ([(18)F]FDG) and 3'-deoxy-3'-[(18)F]fluorothymidine ([(18)F]FLT), single photon emission tomography (SPECT) with [(99m)Tc]TcO4 ([(99m)Tc]TEC), X-ray computed tomography, and fluorescent confocal endomicroscopy (FCE) images were acquired during tumor progression in female PyMT mice. Imaging with [(18)F]FDG and [(99m)Tc]TEC was also performed in untreated, doxorubicin-treated, and docetaxel-treated PyMT mice. Total tumor volumes were quantified. Tumors were collected and macroscopic and histological examinations were performed.Results: All PyMT mice developed multifocal tumors of the mammary epithelium that became palpable at 8 weeks of age (W8). Computed tomography (CT) detected tumors at W14, while a clear tumoral uptake of [(99m)Tc]TEC and [(18)F]FDG was present as early as W6 and W8, respectively. No contrast between mammary tumors and surrounding tissue was observed at any stage with [(18)F]FLT. FCE detected an angiogenic switch at W10. Lung metastases were not clearly evidenced by imaging. Doxorubicin and docetaxel treatments delayed tumor growth, as shown by [(18)F]FDG and [(99m)Tc]TEC, but tumor growth resumed upon treatment discontinuation. Tumor growth fitted an exponential model with time constant rates of 0.315, 0.145, and 0.212 week(-1) in untreated, doxorubicin, and docetaxel groups, respectively.Conclusions: Molecular imaging of mammary tumors in PyMT is precocious, precise, and predictive. [(18)F]FDG-PET and [(99m)Tc]TEC SPECT monitor tumor response to chemotherapy. [ABSTRACT FROM AUTHOR]
Databáze:	Complementary Index
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