| Autor: |
Das, Debashree, Bose, Anupama, Chattopadhyay, Parimal, Ray, Lalitagauri |
| Předmět: |
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| Zdroj: |
Indian Chemical Engineer; Jun2016, Vol. 58 Issue 2, p118-135, 18p |
| Abstrakt: |
A yeast strain isolated from soil and identified asCryptococcus laurentiiwas found capable of producing thermostable extracellular invertase. The extracellular enzyme was thermostable, and the optimum temperature, pH and time for enzyme-substrate reaction were found to be 60°C, 5.5 and 5 min, respectively. Mn2+stimulated the activity of enzyme significantly while Co2+, Ca2+, Cu2+and Fe3+showed little stimulatory effects. Cetyltrimethylammonium bromide showed stimulatory and ethylenediaminetetraacetic acid showed inhibitory effects on enzyme activity. Enzyme was found to be thermostable up to 60°C at pH 5.5. The maximum invertase production (35.32 IU ml−1) was achieved at 30°C, 120 rpm and pH 4.5 on 48 h using 1.0% cell suspension (24 h old) as inoculum in 50 ml medium in 250 ml Erlenmeyer flask. The superior carbon and nitrogen sources for the production of invertase were found to be sucrose (2.0%), peptone (nitrogen level 1 mg/ml). TheKmandVmaxwere 2.9 mg ml−1and 39.7 mg min−1ml−1, respectively. The maximum enzyme activity obtained was 44.72. [ABSTRACT FROM PUBLISHER] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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