| Autor: |
Kulkarni, Dinraj, Shah, Amisha A., Ingle, Yashawant, Ramaiah, Varadhrajula Venkat, Goswamy, Kamal, Naz-Muddebihal, Farah, Syed, Shibli S., Kulkarni, Apurva |
| Předmět: |
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| Zdroj: |
Journal of International Oral Health; Apr2016, Vol. 8 Issue 4, p422-428, 7p, 5 Color Photographs, 1 Diagram, 1 Chart, 2 Graphs |
| Abstrakt: |
Background: Increase evidence indicates that lymphangiogenesis in cancer is an important factor in promoting tumor progression and lymphatic vessels are major routes for metastasis in head and neck squamous cell carcinoma (HNSCC), but lymphatic endothelial cells (LCEs) are difficult to recognize in tumor histological section. Of all recent markers, D2-40 stains podoplanin, a molecule expressed in LEC's but its prognostic usefulness in HNSCC is not so well understood. In this study, we aimed to investigate immunohistochemical expression of D2-40 in different stages and grades of oral SCC (OSCC) and also studied lymphatic vessel density (LVD) both intratumorally and peritumorally to determine its prognostic value. Materials and Methods: A total of 50 cases of HNSCC were stained for D2-40. LVD was assessed both intratumorally as well as peritumorally by counting positive reaction in 10 hot spot areas at ×100 magnification. Results: D2-40 was specific for LEC's and, therefore, stained only lymphatic vessels. Peritumoral lymphatic vessel (PTLV) was more numerous and larger than intratumoral lymphatic vessel (ITLV). Statistically significant differences were observed between PTLV density (PTLVD) and ITLV density (ITLVD). PTLVD was found to be more significantly associated with higher grades of OSCC. Tumor D2-40 staining was positively associated with lymphatic vessel invasion. Conclusion: D2-40 can be considered as an ideal lymphatic marker and LVD is an important parameter to predict the prognosis of HNSCC. PTLVD and D2-40 positive tumor cells are strongly associated with metastasis and other clinical parameters of worse prognosis. D2-40 may play a vital role in deciding on therapeutic strategies for the patient with oral cancer. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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