| Abstrakt: |
A soluble protein extract, obtained after [SUP35]S labeling exponential cultures of Cryptothecodinium cohnii for 4-5 generations, was fractionated on DNA-cellulose. The protein eluted at high salt concentrations from this homologous DNA-containing matrix was then separated by chromatography on BioGel P150 and P200 sieve gels. Two prominent chromatographic species of 22,000 and 35,000 daltons plus a substantial amount of material in the molecular weight range of 80,000-140,000 were resolved. The application of this analytical procedures to the study of transcriptional control in the cell cycle is discussed. [ABSTRACT FROM AUTHOR] |
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