KOTMIN13, a Korean herbal medicine alleviates allergic inflammation in vivo and in vitro.

Autor:	Eujin Lee, Sun-Gun Kim, Na-Young Park, Hyo-Hyun Park, Kyu-Tae Jeong, Jongkeun Choi, In-Hae Lee, Hwadong Lee, Keuk-Jun Kim, Eunkyung Lee
Předmět:	ASTHMA treatment PHYTOTHERAPY THERAPEUTIC use of plant extracts LUNG anatomy IMMUNOGLOBULIN analysis ANAPHYLAXIS ANIMAL experimentation ASTHMA BIOLOGICAL models BRONCHOALVEOLAR lavage CYTOKINES ENZYME-linked immunosorbent assay HIGH performance liquid chromatography HISTOLOGICAL techniques MAST cells MICE PLETHYSMOGRAPHY PROBABILITY theory RESEARCH funding SPECTROPHOTOMETRY DESCRIPTIVE statistics LEUKOCYTE count IN vitro studies ONE-way analysis of variance IN vivo studies
Zdroj:	BMC Complementary & Alternative Medicine; 6/6/2016, Vol. 16, p1-10, 10p, 1 Color Photograph, 1 Chart, 4 Graphs
Abstrakt:	Background: The ethanol extract of KOTMIN13, composed of Flowers, Semen, Inula japonica Trichosanthes kirilowii Peucedanum praeruptorum Radix, and Bulbs, was investigated for its anti-asthmatic and Allium macrostemon anti-allergic activities. Methods: The anti-asthmatic effects of KOTMIN13 were evaluated on ovalbumin (OVA)-induced murine asthma model. Anti-allergic properties of KOTMIN13 in bone-marrow derived mast cells (BMMC) and passive cutaneous anaphylaxis (PCA) in vivo were also examined. Results: In asthma model, KOTMIN13 effectively suppressed airway hyperresponsiveness induced by aerosolized methacholine when compared to the levels of OVA-induced mice. KOTMIN13 treatment reduced the total leukocytes, eosinophil percentage, and Th2 cytokines in the bronchoalveolar lavage fluids in OVA-induced mice. The increased levels of eotaxin and Th2 cytokines in the lung as well as serum IgE were decreased by KOTMIN13. The histological analysis shows that the increased inflammatory cell infiltration and mucus secretion were also reduced. In addition, the degranulation and leukotriene C4 production were inhibited in BMMC with IC50 values of 3.9 µg/ml and 1.7 µg/ml, respectively. Furthermore, KOTMIN13 treatment attenuated mast-mediated PCA reaction. Conclusions: These results demonstrate that KOTMIN13 has anti-asthmatic and anti-allergic effects in vivo and in vitro models. [ABSTRACT FROM AUTHOR]
Databáze:	Complementary Index
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