| Autor: |
IONESCU, Mihaela, HINESCU, G. Lavinia, RĂDULESCU, S. Flavian, MIRON, S. Dalia, POSTOARCĂ, G. Angela, COSMESCU, Cristiana, RANETTI, M. Cristina, MIRCIOIU, Constantin |
| Zdroj: |
Balkan Military Medical Review; 2014 Supplement, Vol. 17, p230-230, 1p |
| Abstrakt: |
THE AIM was to elaborate a protective gel with DTPA-Ca/Zn known as chelators for radioactive transuranic elements. MATERIALS AND METHODS. The in vitro release studies were performed using a static system with 6 diffusion vertical cells for gels and hydrophilic/hydrophobic membranes. Carbopol 934 gels were prepared with 2% DTPA Ca/Zn microemulsions of decorporation agents. The receiver medium was phosphate buffer pH 7.4 maintained at 32°C and continuously stirred at 600 rpm. Approximately 300mg of gel was loaded on the surface of the membrane and covered with a glass disk. Samples (0.5ml) were taken from receiving solution at 15, 30, 60, 120, 180, 240, 300 and 360 minutes and replaced with same volume of receiving medium to maintain a constant volume. A spectrophotometric method based on the complexation reaction with FeCl3/HCl in phosphate buffer was used for the determination of DTPA Ca/Zn concentrations from the receptor fl uid. Results. The release experiments for DPTA Ca/Zn microemulsions were governed by the diffusion laws. In all cases, the average data release were fi tted by the fi rst degree equation with 0,99 - correlation coeffi cient. The average released amount (μg/cm2*t1/2) had reached the same level in both types of membranes: DTPA-Zn 25; DTPA-Ca 111. Conclusions. The gels transfer was continuous, at a constant rate, but slowly. After six hours it did not reached the saturation point. Including the two decorporation agents in microemulsion gels had a retarding effect of released amount. Administration of this topic formulation can lead to an extension of the in vivo effect. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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