| Autor: |
Casper C. Hoogenraad, Phebe Wulf, Natalia Schiefermeier, Tatiana Stepanova, Niels Galjart, J.Victor Small, Frank Grosveld, Chris I. de Zeeuw, Anna Akhmanova |
| Předmět: |
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| Zdroj: |
EMBO Journal; 11/17/2003, Vol. 22 Issue 22, p6004, 12p |
| Abstrakt: |
Bicaudal D is an evolutionarily conserved protein, which is involved in dynein-mediated motility both in Drosophila and in mammals. Here we report that the N-terminal portion of human Bicaudal D2 (BICD2) is capable of inducing microtubule minus end-directed movement independently of the molecular context. This characteristic offers a new tool to exploit the relocalization of different cellular components by using appropriate targeting motifs. Here, we use the BICD2 N-terminal domain as a chimera with mitochondria and peroxisome-anchoring sequences to demonstrate the rapid dynein-mediated transport of selected organelles. Surprisingly, unlike other cytoplasmic dynein-mediated processes, this transport shows very low sensitivity to overexpression of the dynactin subunit dynamitin. The dynein-recruiting activity of the BICD2 N-terminal domain is reduced within the full-length molecule, indicating that the C-terminal part of the protein might regulate the interaction between BICD2 and the motor complex. Our findings provide a novel model system for dissection of the molecular mechanism of dynein motility. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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