| Autor: |
Brooks, Ashley, Creighton, Erica K., Gandolfi, Barbara, Khan, Razib, Grahn, Robert A., Lyons, Leslie A. |
| Předmět: |
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| Zdroj: |
Journal of Forensic Sciences; May2016, Vol. 61 Issue 3, p594-606, 13p |
| Abstrakt: |
Phenotypic and genotypic characteristics of the cat can be obtained from single nucleotide polymorphisms ( SNPs) analyses of fur. This study developed miniplexes using SNPs with high discriminating power for random-bred domestic cats, focusing on individual and phenotypic identification. Seventy-eight SNPs were investigated using a multiplex PCR followed by a fluorescently labeled single base extension ( SBE) technique ( SNaPshot®). The SNP miniplexes were evaluated for reliability, reproducibility, sensitivity, species specificity, detection limitations, and assignment accuracy. Six SNPplexes were developed containing 39 intergenic SNPs and 26 phenotypic SNPs, including a sex identification marker, ZFXY. The combined random match probability ( cRMP) was 6.58 × 10−19 across all Western cat populations and the likelihood ratio was 1.52 × 1018. These SNPplexes can distinguish individual cats and their phenotypic traits, which could provide insight into crime reconstructions. A SNP database of 237 cats from 13 worldwide populations is now available for forensic applications. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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