| Autor: |
Feixue Li, Xiaoping Miao, Yonglong Chen, Curry Jr., Thomas E. |
| Předmět: |
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| Zdroj: |
Reproduction, Fertility & Development; 2016, Vol. 28 Issue 6, p742-749, 8p |
| Abstrakt: |
CXADR-like membrane protein (CLMP) is a novel cell-cell adhesion molecule. The present study investigated the spatiotemporal expression pattern of CLMP and its regulation in the rat ovary during the periovulatory period. Real-time polymerase chain reaction analysis revealed that Clmp mRNA was rapidly stimulated in intact ovaries by 4 h after human chorionic gonadotrophin (hCG) treatment. In situ hybridisation analysis demonstrated that Clmp mRNA expression was stimulated in theca cells at 4 h after hCG and remained elevated until 12 h. Clmp mRNA was also upregulated in granulosa cells and was present in forming corpora lutea. Our data indicate that the protein kinase A but not the protein kinaseCpathway regulates the expression of ClmpmRNAin granulosa cells. Phosphatidylinositol 3 kinase and p38 kinase are also involved in regulating ClmpmRNA expression. The stimulation of ClmpmRNA by hCG requires new protein synthesis. Furthermore, inhibition of epidermal growth factor receptor activation significantly inhibited Clmp mRNA expression, whereas inhibition of prostaglandin synthesis or progesterone action had no effect. The stimulation of CLMP in the rat ovarymay be important in cell adhesion events during ovulation and luteal formation such as maintaining the structure and communication of ovarian follicular and luteal cells. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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