| Autor: |
Xu, Jianbin, Li, Jinming, Lin, Sien, Wu, Tianyi, Huang, Heqin, Zhang, Kunyu, Sun, Yuxin, Yeung, Kelvin W. K., Li, Gang, Bian, Liming |
| Předmět: |
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| Zdroj: |
Advanced Functional Materials; 4/19/2016, Vol. 26 Issue 15, p2463-2472, 10p |
| Abstrakt: |
Cartilage loss is a leading cause of disability among adults, and effective therapy remains elusive. Human mesenchymal stem cells (hMSCs), which have demonstrated self-renewal and multipotential differentiation, are a promising cell source for cartilage repair. However, the hypertrophic differentiation of the chondrogenically induced MSCs and resulting tissue calcification hinders the clinical translation of MSCs for cartilage repair. Here, a multifunctional nanocarrier based on quantum dots (QDs) is developed to enhance chondrogenic differentiation and suppress hypertrophy of hMSCs simultaneously. Briefly, the QDs are modified with β-cyclodextrin (β-CD) and RGD peptide. The resulting nanocarrier is capable of carrying hydrophobic small molecules such as kartogenin in the hydrophobic pockets of conjugated β-CD to induce chondrogenic differentiation of hMSCs. Meanwhile, via electrostatic interaction the conjugated RGD peptides bind the cargo siRNA targeting Runx2, which is a key regulator of hMSC hypertrophy. Furthermore, due to the excellent photostability of QDs, hMSCs labeled with the nanocarrier can be tracked for up to 14 d after implantation in nude mice. Overall, this work demonstrates the potential of our nanocarrier for inducing and maintaining the chondrogenic phenotype and tracking hMSCs in vivo. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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