| Autor: |
Baojie Jiang, Ruiqin Zhang, Dan Feng, Fangzhong Wang, Kuimei Liu, Yi Jiang, Kangle Niu, Quanquan Yuan, Mingyu Wang, Hailong Wang, Youming Zhang, Xu Fang |
| Předmět: |
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| Zdroj: |
Frontiers in Microbiology; 4/12/2016, p1-9, 9p |
| Abstrakt: |
The lack of selective markers has been a key problem preventing multistep genetic engineering in filamentous fungi, particularly for industrial species such as the lignocellulose degrading Penicillium oxalicum JUA10-1(formerly named as Penicillium decumbens). To resolve this problem, we constructed a genetic manipulation system taking advantage of two established genetic systems: the Cre-loxP system and Tet-on system in P. oxalicum JUA10-1. This system is efficient and convenient. The expression of Cre recombinase was activated by doxycycline since it was controlled by Tet-on system. Using this system, two genes, ligD and bglI, were sequentially disrupted by loxP flanked ptrA. The successful application of this procedure will provide a useful tool for genetic engineering in filamentous fungi. This system will also play an important role in improving the productivity of interesting products and minimizing by-product when fermented by filamentous fungi. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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