| Autor: |
Buhr, T.L., Young, A.A., Bensman, M., Minter, Z.A., Kennihan, N.L., Johnson, C.A., Bohmke, M.D., Borgers ‐ Klonkowski, E., Osborn, E.B., Avila, S.D., Theys, A.M.G., Jackson, P.J. |
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| Zdroj: |
Journal of Applied Microbiology; Apr2016, Vol. 120 Issue 4, p1074-1084, 11p, 1 Color Photograph, 3 Diagrams, 1 Chart, 2 Graphs |
| Abstrakt: |
Aim To develop test methods and evaluate survival of Bacillus thuringiensis kurstaki cry− HD-1 and B. thuringiensis Al Hakam spores after exposure to hot, humid air inside of a C-130 aircraft. Methods and Results Bacillus thuringiensis spores were either pre-inoculated on 1 × 2 or 2 × 2 cm substrates or aerosolized inside the cargo hold of a C-130 and allowed to dry. Dirty, complex surfaces (10 × 10 cm) swabbed after spore dispersal showed a deposition of 8-10 log10 m−2 through the entire cargo hold. After hot, humid air decontamination at 75-80°C, 70-90% relative humidity for 7 days, 87 of 98 test swabs covering 0·98 m2, showed complete spore inactivation. There was a total of 1·67 log10 live CFU detected in 11 of the test swabs. Spore inactivation in the 98 test swabs was measured at 7·06 log10 m−2. Conclusions Laboratory test methods for hot, humid air decontamination were scaled for a large-scale aircraft field test. The C-130 field test demonstrated that hot, humid air can be successfully used to decontaminate an aircraft. Significance and Impact of the Study Transition of a new technology from research and development to acquisition at a Technology Readiness Level 7 is unprecedented. [ABSTRACT FROM AUTHOR] |
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