Optimization of the Electrospray Ionization Source with the Use of the Design of Experiments Approach for the LC–MS-MS Determination of Selected Metabolites in Human Urine.

Autor:	Szerkus, Oliwia, Yumba Mpanga, Arlette, Markuszewski, Michat J., Kaliszan, Roman, Siluk, Danuta
Předmět:	EXPERIMENTAL design MULTIVARIATE analysis MATHEMATICAL optimization DESORPTION electrospray ionization ELECTROSPRAY ionization mass spectrometry
Zdroj:	LC-GC North America; Mar2016 Supplement Current Trends in Mass Spectrometry, Vol. 34, p8-16, 9p
Abstrakt:	Simultaneous quantification of metabolites in biological fluids, where they are present at different concentration levels, is usually a challenging analytical task. One of the steps that should be undertaken to increase analytical method efficiency is optimization of the electrospray ionization (ESI) source, which can be especially helpful in increasing method sensitivity of agents with poor ionization characteristics. We present here a step-by-step ESI optimization strategy with the use of the design of experiments (DoE) approach. This multivariate statistical approach allows effective evaluation of the effects of multiple factors and interactions among factors on a given response in a minimum number of experimental runs. [ABSTRACT FROM AUTHOR]
Databáze:	Complementary Index
Externí odkaz:	Zobrazit plný text záznamu