| Autor: |
Sellner, Kevin G., Lyons, Larry, Perry, Elgin S., Heimark, Douglas B. |
| Předmět: |
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| Zdroj: |
Journal of Phycology; Mar1982, Vol. 18 Issue 1, p142-148, 7p |
| Abstrakt: |
Exponeutially growing cultures of Thalassiosira Huviatilis Hustedt and Dunaliella tertiolecta Butcher were exposed to 4 mm temperature shocks of 5° to 20°C above ambient (20°C). Photosynethetic carbon fixation, changes in in vivoe fluorescence and fluorescence on the addition of the herbicide DCMD (3-3, 4-dichlosophenyl)-1, 1-dimethylurea) were measured over the subsequent 24 h. The fluorescence ratio (R, DCMU-enhanced fluorescencelin vivo fluorescence) paralleled changes in phtosynthesis over this period; both were significantly reduced (P<0.05) by temperature shocks of +5° and +20°C, but +5° and +10°C treatments had no inhibitory effect on either relative to the control. The instantaneous response obtained with fluorescence ratio indicates that the technique might be applicable to routine bioassay procedures and thus replace the time consuming methods none used for the estimation of [sup14]C-incorporation and growth rates. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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