| Autor: |
Birkin, J.A., Fry, J.R., Millard, L.G. |
| Předmět: |
|
| Zdroj: |
Clinical & Experimental Dermatology; Mar1991, Vol. 16 Issue 2, p98-105, 8p |
| Abstrakt: |
Protein and N-linked glycoprotein biosynthesis was studied in the uninvolved epidermis of patients with psoriasis by the incorporation of radiolabelled leucine and mannose prior to and during PUVA treatment. Analysis of the polyacrylamide gel electrophoresis (PAGE) patterns of the 3H-labelled proteins and glycoproteins showed that the major changes in untreated uninvolved psoriatic epidermis compared to normal epidermis were: (a) a shift towards the synthesis of low-molecular-weight glycoproteins; (b) the absence of a 48-kDa peak labelled with mannose; (c) the appearance of 3H-mannose-labelled peaks at 40-36 kDa. PUVA treatment gradually changed the PAGE profile back more towards that expected for normal epidermis, with the reintroduction of a 52-48-kDa region. This effect was dependent on uninterrupted treatment. the PUVA-treated PAGE profiles were compared to those expected in skin tumours (i.e. increased 3H-mannose-labelled peaks at 95 and 40-34 kDa with an absence of 62-kDa peaks). It appeared that these criteria were not seen generally as a result of PUVA treatment. However, the results indicate that tumour development may be possible if a patients responds to PUVA treatment by showing an increased peak at 95 and 40-34 kDa in association with a loss of an 3H-mannose-labelled peak at 62 kDa. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
Plný text