| Autor: |
Espinoza-Mellado, María del, Reyes-Picaso, Carolina, Garcés-Pérez, Miriam, Jardón-Serrano, Cynthia, López-Villegas, Edgar, Giono-Cerezo, Silvia |
| Předmět: |
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| Zdroj: |
Archives of Microbiology; Mar2016, Vol. 198 Issue 2, p199-204, 6p |
| Abstrakt: |
The MAP-LC3 system regulates the intracellular formation of autophagy-associated vacuoles. These vacuoles contain the LC3 protein; thus it has been utilized as a marker to identify autophagosomes. The aim of our study was to investigate whether Haemophilus influenzae strains and their supernatants could activate autophagy in human larynx carcinoma cell line (HEp-2). We demonstrate that higher expression of the LC3B-II protein was induced, particularly by nontypeable Haemophilus influenzae (NTHi) 49766 and by supernatants, containing <50 kDa proteins, of both strains. Ultrastructural studies demonstrate vacuoles with a double membrane and/or membrane material inside, showing similar features to those of autophagic vacuoles. Together, our findings demonstrate that H. influenzae strains and their supernatants trigger an autophagic process. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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