| Autor: |
Stein, Ofer, Damari‐Weissler, Hila, Secchi, Francesca, Rachamilevitch, Shimon, German, Marcelo A., Yeselson, Yelena, Amir, Rachel, Schaffer, Arthur, Holbrook, N. Michele, Aloni, Roni, Zwieniecki, Maciej A., Granot, David |
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| Zdroj: |
New Phytologist; Mar2016, Vol. 209 Issue 4, p1484-1495, 12p, 2 Color Photographs, 3 Diagrams, 7 Graphs |
| Abstrakt: |
Plants have two kinds of fructokinases ( FRKs) that catalyze the key step of fructose phosphorylation, cytosolic and plastidic. The major cytosolic tomato FRK, Sl FRK2, is essential for the development of xylem vessels., In order to study the role of Sl FRK3, which encodes the only plastidic FRK, we generated transgenic tomato ( Solanum lycopersicon) plants with RNAi suppression of Sl FRK3 as well as plants expressing beta-glucoronidase ( GUS) under the Sl FRK3 promoter., GUS staining indicated SlFRK3 expression in vascular tissues of the leaves and stems, including cambium, differentiating xylem, young xylem fibers and phloem companion cells. Suppression of Sl FRK3 reduced the stem xylem area, stem and root water conductance, and whole-plant transpiration, with minor effects on plant development. However, suppression of Sl FRK3 accompanied by partial suppression of Sl FRK2 induced significant growth-inhibition effects, including the wilting of mature leaves. Grafting experiments revealed that these growth effects are imposed primarily by the leaves, whose petioles had unlignified, thin-walled xylem fibers with collapsed parenchyma cells around the vessels. A cross between the Sl FRK2-antisense and Sl FRK3- RNAi lines exhibited similar wilting and anatomical effects, confirming that these effects are the result of the combined suppression of Sl FRK3 and Sl FRK2., These results demonstrate a role of the plastidic Sl FRK3 in xylem development and hydraulic conductance. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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