| Autor: |
Côco, Hariane, Pernomian, Larissa, Marchi, Katia C., Gomes, Mayara S., Andrade, Cláudia R., Ramalho, Leandra N. Z., Tirapelli, Carlos R., Oliveira, Ana M. |
| Předmět: |
|
| Zdroj: |
Journal of Pharmacy & Pharmacology; Jan2016, Vol. 68 Issue 1, p63-75, 13p |
| Abstrakt: |
Objectives Our main objective was to investigate the mechanisms underlying the effects of hyperhomocysteinaemia ( HHcy) on contractile response mediated by α1-adrenoceptors in the rat corpus cavernosum. Methods Concentration-response curves for phenylephrine ( PE) were obtained in strips of corpus cavernosum, in absence or after incubation with tiron, tempol or polyethylene glycol ( PEG)-catalase combined or not with tempol. We also measured the superoxide anion ( O2−) and hydrogen peroxide ( H2O2) generation, superoxide dismutase ( SOD) and catalase activity and α-actin expression in rat corpus cavernosum from both groups. Key findings HHcy increased PE-induced contraction in cavernosal strips. Tiron, PEG-catalase or tempol increased PE-induced contraction in strips from control rats, but it was not altered by tiron or PEG-catalase in HHcy rats, whereas tempol reduced this response. The combination of PEG-catalase and tempol did not alter the contractile response to PE in both groups. HHcy increased O2− generation and SOD activity, whereas H2O2 concentration was reduced. Finally, HHcy did not alter catalase activity or expression of α-actin. Conclusions The major new finding from this study is that HHcy induced a marked increase in PE-induced contraction in rat corpus cavernosum by a mechanism that involves increased O2− generation and it could play a role in the pathogenesis of erectile dysfunction associated with HHcy. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
Plný text