Exercise reverses OVA-induced inhibition of glucocorticoid receptor and increases anti-inflammatory cytokines in asthma.
Autor: | Silva, R. A., Almeida, F. M., Olivo, C. R., Saraiva‐Romanholo, B. M., Martins, M. A., Carvalho, C. R. F. |
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Předmět: |
ASTHMA treatment
AEROBIC exercises ANALYSIS of variance ANIMAL experimentation ASTHMA BIOLOGICAL models CELL adhesion molecules CELL receptors EOSINOPHILS GLUCOCORTICOIDS DIGITAL image processing IMMUNOHISTOCHEMISTRY INTERLEUKINS LUNGS MICE MICROSCOPY PROBABILITY theory RESEARCH funding STATISTICS DNA-binding proteins DATA analysis VASCULAR endothelial growth factors DESCRIPTIVE statistics |
Zdroj: | Scandinavian Journal of Medicine & Science in Sports; Jan2016, Vol. 26 Issue 1, p82-92, 11p, 1 Color Photograph, 1 Diagram, 4 Graphs |
Abstrakt: | The purpose of this study was to determine the effect of aerobic exercise training (AT) on the expression of glucocorticoid receptors (GR) and anti-inflammatory cytokines in an asthma model. BALB/c mice were divided into groups control (CT; nonsensitized/nontrained), aerobic training (AT; nonsensitized/trained), ovalbumin (OVA; sensitized/not trained), and OVA+AT (sensitized/ trained). OVA groups received OVA by inhalation, and the AT groups completed 1, 3, or 7 days of exercise (60 min/session). Expression of GR, IL-4, IL-5, IL-10, IL-1ra, NF-κB, TGF-β, VEGF, ICAM-1, VCAM-1; eosinophils counting; and airway remodeling (AR) features [airway smooth muscle (ASM) and epithelial thickness and collagen fiber deposition] were quantified. OVA sensitization induced a decrease in the expression of GR and increases in the eosinophil, IL-4, IL-5, NF-κBB, TGF-β, VEGF, ICAM-1, VCAM-1, and AR features (P < 0.05). After 3 days, AT reversed the OVA-induced reduction in the expression of GR, and subsequently induced increases in the expression of IL-10 and IL-1ra (seventh day). In contrast, the eosinophil migration, the expression of NF-κBB, IL-4, IL-5, TGF-β, RANTES, VEGF, ICAM-1, VCAM-1, and the AR features (P < 0.05) were reduced. AT increases the expression of GR and anti-inflammatory cytokines (IL-10 and IL-1ra) and reduces the expression of inflammatory mediators and airway inflammation in an animal model of asthma. [ABSTRACT FROM AUTHOR] |
Databáze: | Complementary Index |
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