Pathological study of chronic pulmonary toxicity induced by intratracheally instilled Asian sand dust (Kosa): possible association of fibrosis with the development of granulomatous lesions.

Autor:	Akinori Shimada, Yukari Kohara, Misaki Naota, Yoshimi Kobayashi, Takehito Morita, Kenichiro Inoue, Hirohisa Takano
Předmět:	CHRONIC disease diagnosis LUNG disease diagnosis FIBROSIS ANIMAL experimentation CELL physiology COLLAGEN ECOLOGY GRANULOMA IMMUNOGLOBULINS IMMUNOHISTOCHEMISTRY INTERLEUKINS LYMPH nodes MACROPHAGES MEDICAL protocols MICE POLYMERS SILICA T-test (Statistics) CD4 antigen DATA analysis CONTROL groups DESCRIPTIVE statistics DIAGNOSIS
Zdroj:	Folia Histochemica et Cytobiologica; 2015, Vol. 53 Issue 4, p294-306, 13p
Abstrakt:	Exposure to Asian sand dust (ASD) is associated with enhanced pulmonary morbidity and mortality, and the reporting of such cases has rapidly increased in East Asia since 2000. The purpose of the study was to assess chronic lung toxicity induced by ASD.~Introduction~Background~A total of 174 ICR mice were randomly divided into 5 control and 17 exposure groups. Suspensions of low dose (0.2, 0.4 mg) and high dose (3.0 mg) of ASD particles in saline were intratracheally instilled into ICR mice, followed by sacrifice at 24 hours, 1 week, and 1, 2, 3 and 4 months after instillation. Paraffin sections of lung tissues were stained with hematoxylin and eosin and by immunohistochemistry to detect ¿-smooth muscle actin, collagen III, matrix metalloproteinase-9 (MMP-9), tissue inhibitor of metalloproteinases-1 (TIMP-1), CD3, CD20, immunoglobulin G, interleukin-1ß and inducible nitric oxide synthase.~Material and Methods~Methods~A lung histological examination revealed similar patterns in the lesions of the groups treated with high (3.0 mg) or low dose (0.4 mg) of ASD. Acute inflammation was observed 24 h after treatment and subsided after 1 week; persistent granulomatous changes were observed at 2 months, focal lymphocytic infiltration at 3 months, and granuloma formation at 4 months. An increase in the size of granulomatous lesions was observed over time and was accompanied by collagen deposition in the lesions. The cytoplasm of macrophages in inflammatory lesions showed positive immunolabeling for MMP-9 at 24 h, 1 and 2 months after instillation of 3.0 mg of ASD. Positive immunolabeling for TIMP-1 was demonstrated in the cytoplasm of macrophages at 2 and 4 months after instillation of 3.0 mg of ASD. These findings suggest association between the expression of MMP-9 and TIMP-1 with the development of lung granulomatous lesions.~Results~Results~These findings suggest that collagen deposition resulting from the altered regulation of extracellular matrix is associated with granuloma formation in the lungs of mice treated with ASD.~Conclusions~Conclusions [ABSTRACT FROM AUTHOR]
Databáze:	Complementary Index
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