| Autor: |
ADU, K. T., KAYODE, R. M. O., ADU, M. D. |
| Předmět: |
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| Zdroj: |
Ethiopian Journal of Environmental Studies & Management; 2015 Suppl. 1, Vol. 8, p738-751, 14p |
| Abstrakt: |
This experiment reports the physicochemical characterization of cellulose produced by Kurthia gibsonii CAC1 isolated from cassava dumpsites in Ibadan, Nigeria. Lineweaver-Burk plot of cellulose activities of K. gibsonii CAC1 was examined with Kmax and Vmax of the enzyme assayed. At optimal pH of 5.0, 10% increase in enzymatic activities was observed. The enzymatic activities of K. gibsonii CAC1 were optimal at 30°C and were still stable at 60°C with 50% reduction in cellulose activities. Cellulose activities of K. Gibsonii CAC1 showed significant difference (p≥0.05) with the different cations used. There was no significant difference (p≤0.05) observed with increased concentrations (p≤0.05) using two-way ANOVA. Ca2+ highly enhanced the cellulose activities of K.gibsonii CAC1 by 60% at lOmM. Highest inhibition by Hg2+ was observed at 20mM with 50% inhibition. At increasing concentration of the inhibitors, there was no significant difference (p≤0.05) in cellulose activities; although the effect of each inhibitor on the enzymatic activity was significantly different (p≥0.05). Benzoic acid gave the highest inhibition of the cellulose activities in K. gibsonii CAC1 by 30% at 20mM, while Ethylene diamine-tetraacetic acid (EDTA) boosted the enzymatic activities by 10% at lOmM. There was a significant difference (p≥0.05) in the effect of different surfactants on the enzymatic activity but no significant difference (p≤0.05) with the concentration of the surfactants on cellulose activity. Enzymatic activities of the bacterium were enhanced by Polyoxyethylenesorbitan mono-oleate (Tween 80) with a boost of 50%. Increasing the concentration of sodium dodecyl sulphate (SDS) and Polyethylene glycol p-isooctylphenylether (Trition X-100) caused a 70% increase in cellulose activities. Cellulose of K. gibsonii CAC1 had Kmax of 7.4 X 10-2 mg/ml and Vmax of 6.7 X 10-1 μg/sec. The cellulose described in this work have many properties that are similar to those obtained from other microbial sources and may be useful for various industrial applications. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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