| Autor: |
Akhter, Shamim, Ansari, Muhammad Sajjad, Rakha, Bushra Allah, Aziz, Tahir, Hassan Andrabi, Syed Murtaza, Ullah, Nemat, Anwar, Muhammad, Qayyum, Mazhar |
| Zdroj: |
Pakistan Journal of Zoology; 2015, Vol. 47 Issue 6, p1645-1648, 4p |
| Abstrakt: |
This study was designed to compare six different milk based extenders for the liquid storage of Nili- Ravi buffalo bull semen. Semen was collected from three Nili-Ravi buffalo bulls with artificial vagina (42°C) at weekly intervals (n=5). Semen was diluted (at 37°C) with six milk based extenders; homogenized milk (HOM), whole buffalo milk (BUF), whole cow milk (COW), whole camel milk (CAM), modified homogenized milk (MOD) and skimmed milk (SKIM). After dilution, semen was cooled to 5°C in 2 h and then it was refrigerated at 5°C for 96 h. Sperm motility (%) and normal acrosomal ridge (NAR; %) were recorded at 24 h intervals till 96 h. Sperm motility did not differ up to 72 h of storage in all experimental extenders, while higher (P<0.05) motility was observed in SKIM after 96 h of storage, that remained similar to sperm motility in HOM extender. Overall least square mean of sperm motility for SKIM was higher (P < 0.05) compared to HOM, BUF, COW, CAM and MOD after 96 h of storage period. Percentage of sperm with NAR remained similar (P>0.05) in SKIM, COW, BUF and HOM extenders at 24 h of storage. However, at 72 and 96 h of storage, values for NAR were higher (P<0.05) in SKIM and COW compared to HOM, BUF, CAM and MOD extenders. The values of NAR in terms of least square means remained higher (P<0.05) for SKIM and COW extenders compared to other extenders. In conclusion, skimmed milk based extender was found superior for chilled buffalo semen stored at 5°C. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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