Expression Profiling after Prolonged Experimental Febrile Seizures in Mice Suggests Structural Remodeling in the Hippocampus.

Autor: Jongbloets, Bart C., van Gassen, Koen L. I., Kan, Anne A., Olde Engberink, Anneke H. O., de Wit, Marina, Wolterink-Donselaar, Inge G., Groot Koerkamp, Marian J. A., van Nieuwenhuizen, Onno, Holstege, Frank C. P., de Graan, Pierre N. E.
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Zdroj: PLoS ONE; 12/18/2015, Vol. 10 Issue 12, p1-24, 24p
Abstrakt: Febrile seizures are the most prevalent type of seizures among children up to 5 years of age (2–4% of Western-European children). Complex febrile seizures are associated with an increased risk to develop temporal lobe epilepsy. To investigate short- and long-term effects of experimental febrile seizures (eFS), we induced eFS in highly febrile convulsion-susceptible C57BL/6J mice at post-natal day 10 by exposure to hyperthermia (HT) and compared them to normotherm-exposed (NT) mice. We detected structural re-organization in the hippocampus 14 days after eFS. To identify molecular candidates, which entrain this structural re-organization, we investigated temporal changes in mRNA expression profiles eFS 1 hour to 56 days after eFS. We identified 931 regulated genes and profiled several candidates using in situ hybridization and histology at 3 and 14 days after eFS. This is the first study to report genome-wide transcriptome analysis after eFS in mice. We identify temporal regulation of multiple processes, such as stress-, immune- and inflammatory responses, glia activation, glutamate-glutamine cycle and myelination. Identification of the short- and long-term changes after eFS is important to elucidate the mechanisms contributing to epileptogenesis. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index