| Autor: |
Jiamsakul, Awachana, Chaiwarith, Romanee, Durier, Nicolas, Sirivichayakul, Sunee, Kiertiburanakul, Sasisopin, Van Den Eede, Peter, Ditangco, Rossana, Kamarulzaman, Adeeba, Li, Patrick CK, Ratanasuwan, Winai, Sirisanthana, Thira |
| Zdroj: |
Journal of Medical Virology; Feb2016, Vol. 88 Issue 2, p234-243, 10p |
| Abstrakt: |
HIV drug resistance assessments and interpretations can be obtained from genotyping (GT), virtual phenotyping (VP) and laboratory-based phenotyping (PT). We compared resistance calls obtained from GT and VP with those from PT (GT-PT and VP-PT) among CRF01-AE and subtype B HIV-1 infected patients. GT predictions were obtained from the Stanford HIV database. VP and PT were obtained from Janssen Diagnostics BVBA's vircoTypeTM HIV-1 and Antivirogram1, respectively. With PT assumed as the "gold standard," the area under the curve (AUC) and the Bland-Altman plot were used to assess the level of agreement in resistance interpretations. A total of 80 CRF01-AE samples from Asia and 100 subtype B from Janssen Diagnostics BVBA's database were analysed. CRF01-AE showed discordances ranging from 3 to 27 samples for GT-PT and 1 to 20 samples for VP-PT. The GT-PT and VP-PT AUCs were 0.76-0.97 and 0.81-0.99, respectively. Subtype B showed 3-61 discordances for GT-PT and 2-75 discordances for VP-PT. The AUCs ranged from 0.55 to 0.95 for GT-PT and 0.55 to 0.97 for VP-PT. Didanosine had the highest proportion of discordances and/or AUC in all comparisons. The patient with the largest didanosine FC difference in each subtype harboured Q151M mutation. Overall, GT and VP predictions for CRF01-AE performed significantly better than subtype B for three NRTIs. Although discrepancies exist, GT and VP resistance interpretations in HIV-1 CRF01-AE strains were highly robust in comparison with the gold-standard PT. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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