| Autor: |
Tsang, Fan Cheung, Po, Lai See, Leung, Ka Man, Lau, Anita, Siu, Wai Yi, Poon, Randy Y.C. |
| Předmět: |
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| Zdroj: |
FEBS Letters; Oct2003, Vol. 553 Issue 3, p277, 9p |
| Abstrakt: |
ING1b can stimulate cell cycle arrest, repair, senescence, and apoptosis. The actions of ING1b are attributed to its activation of the tumor suppressor p53. Here we investigate the more subtle effects of ING1b on the cell cycle and DNA damage responses in the absence of p53. To this end, we have generated isogenic cell lines that expressed ING1b and p53 either individually or in combination under the control of inducible promoters. A five- to 10-fold induction of ING1b over the endogenous protein in a p53-null H1299 background slightly impairs proliferation by increasing the doubling time by ∼10%. Significantly, ectopic expression of ING1b enhanced the G2/M DNA damage checkpoint induced by adriamycin. We demonstrated that the DNA damage-induced cell death mediated by the cooperation between ING1b and p53 was more prominent than by the individual proteins alone. In adriamycin-treated cells, p53 was stabilized and induced the expression of p21CIP1/WAF1, but the expression of ING1b was not affected. The exact targets of ING1b in the p53-null background are not known, but we demonstrated that the transcriptional activities of other members of the p53 family, p63α and p73α, could be activated by ING1b. These data indicate that ING1 has a subtle antiproliferative effect even in the absence of p53, and ING1b enhances the DNA damage responses through p53-dependent and -independent mechanisms. [Copyright &y& Elsevier] |
| Databáze: |
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