| Autor: |
Debruyne, Philip R, Vermeulen, Stefan J, Berx, Geert, Pocard, Marc, Correia da Rocha, Ana-Sofia, Li, Xuedong, Cirnes, Luis, Poupon, Marie-France, van Roy, Frans M, Mareel, Marc M |
| Předmět: |
|
| Zdroj: |
Oncogene; 10/16/2003, Vol. 22 Issue 46, p7199-7208, 10p |
| Abstrakt: |
In subclones of the human colon cancer LoVo cell line, there is a reproducible spontaneous transition from an epithelioid (E) to a round (R) morphotype. The E to R transition is associated with increased cell growth, absence of E-cadherin-dependent compaction in a slow aggregation assay, loss of contact inhibition of motility and directional migration in a wound filling motility assay. Furthermore, none of the E subclones from LoVo was invasive into chick heart fragments. This is in contrast to the R subclones that were either nonadherent or adherent and invasive. Macroarray analysis demonstrated transcriptional downregulation of plakoglobin in R type LoVo cells and this was confirmed at the level of the mRNA by quantitative RT-PCR. Western blotting showed lower expression of all components of the E-cadherin/catenin complex in R subclones. Interestingly, treatment of R subclones with the demethylating agent 5-aza-2'-deoxycytidine resulted in restoration of the E morphotype, higher expression of E-cadherin, but not plakoglobin mRNA, and higher expression of E-cadherin and plakoglobin at the protein level.Oncogene (2003) 22, 7199-7208. doi:10.1038/sj.onc.1206628 [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
