| Autor: |
Daryaei, Amir, Jones, E. Eirian, Alizadeh, Hossein, Glare, Travis R., Falloon, Richard E. |
| Předmět: |
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| Zdroj: |
Biocontrol Science & Technology; Feb2016, Vol. 26 Issue 2, p189-205, 17p |
| Abstrakt: |
Effects of abiotic factors during production (temperature, nutrients, water activity, pH) on conidium fitness (quantity and quality) ofTrichoderma atrovirideLU132 (a key biocontrol agent) were studied. Conidia from the culturing regimes which resulted in greatest and least bioactivity againstRhizoctonia solaniin dual culture assays were selected to assess effects of storage conditions on conidial fitness over time. Further studies assessed interaction effects of temperatures (20°C or 30°C) and sugars (dextrose or sucrose) on conidium germination and bioactivity as fresh conidia, or after 6 months of storage. Biochemical analyses of sugars and fatty acids were carried out to determine relationships between quality variations and cellular characteristics for conidia produced in different culturing conditions. Low trehalose content in conidia (e.g. at 20°C) was associated with the least conidium fitness, although high trehalose content did not necessarily support conidium fitness. High proportions of total fatty acids in conidia were mostly associated with the least conidium fitness. WhenTrichodermawas grown at high carbon to nitrogen ratio (e.g. at C:N 160:1), the total conidium fatty acids content increased. This study also indicated that the monosaccharide dextrose is metabolically optimal forT. atrovirideLU132 at 20°C while the disaccharide sucrose is optimal at 30°C. These studies indicate that physical growth conditions and nutritional requirements attribute in conidium fitness ofT. atrovirideLU132, and provide important knowledge supporting optimum production of biocontrol agents based onT. atroviride, and possibly other similar biocontrol agents. [ABSTRACT FROM PUBLISHER] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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