| Autor: |
Raja, Anju M., Xu, Shuoyu, Zhuo, Shuangmu, Tai, Dean C.S., Sun, Wanxin, So, Peter T.C., Welsch, Roy E., Chen, Chien‐Shing, Yu, Hanry |
| Zdroj: |
Journal of Biophotonics; Oct2015, Vol. 8 Issue 10, p804-815, 12p |
| Abstrakt: |
Cancer initiating cells (CICs) have been the focus of recent anti-cancer therapies, exhibiting strong invasion capability via potentially enhanced ability to remodel extracellular matrices (ECM). We have identified CICs in a human breast cancer cell line, MX-1, and developed a xenograft model in SCID mice. We investigated the CICs' matrix-remodeling effects using Second Harmonic Generation (SHG) microscopy to identify potential phenotypic signatures of the CIC-rich tumors. The isolated CICs exhibit higher proliferation, drug efflux and drug resistant properties in vitro; were more tumorigenic than non-CICs, resulting in more and larger tumors in the xenograft model. The CIC-rich tumors have less collagen in the tumor interior than in the CIC-poor tumors supporting the idea that the CICs can remodel the collagen more effectively. The collagen fibers were preferentially aligned perpendicular to the CIC-rich tumor boundary while parallel to the CIC-poor tumor boundary suggesting more invasive behavior of the CIC-rich tumors. These findings would provide potential translational values in quantifying and monitoring CIC-rich tumors in future anti-cancer therapies. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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