RAPD Analysis of Rosa laevigata Michx. from Different Origins.

Autor: Xiaoling WANG, Xiaoli WANG, Churui CHANG
Předmět:
Zdroj: Agricultural Biotechnology (2164-4993); Oct2015, Vol. 4 Issue 5, p33-36, 4p
Abstrakt: [Objective] This study aimed to investigate the genetic diversity and phylogenetic relationship of 30 populations of Rosa laevigata Michx. from different origins. [Method] Using RAPD molecular marker technique, eight effective primers were screened from35 RAPD random primers for amplification. UPGMA cluster analysis was performed using NTSYSpc. ver. 2.02 software. [ Result] A total of 86 loci were amplified using the screened primers, including 84 polymorphic loci, indicating an average polymorphic percentage (PPB) of 97. 67%. The similarity coefficients of 30 populations of R. laevigata Michx. ranged from 0. 11 to 0.58, ad these populations were clustered into groups A, B and C. [Conclusion] R. laevigata Michx. exhibits multiple genetic polymorphism. R. laevigata Michx. populations from Guizhou Province have closer genetic relationships. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index