1,25-Dihydroxyvitamin D3 and its analogues increase catalase at the mRNA, protein and activity level in a canine transitional carcinoma cell line.

Autor: Middleton, R. P., Nelson, R., Li, Q., Blanton, A., Labuda, J. A., Vitt, J., Inpanbutr, N.
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Zdroj: Veterinary & Comparative Oncology; Dec2015, Vol. 13 Issue 4, p452-463, 12p
Abstrakt: Antioxidant enzymes, such as catalase, superoxide dismutases ( SOD), MnSOD and Cu/ ZnSOD, protect cells by scavenging reactive oxygen species ( ROS). Numerous studies have reported the anti-cancer effects of 1,25-dihydroxyvitamin D3 (calcitriol) and its related analogues, seocalcitol and analogue V. In this study, canine bladder transitional cell carcinoma ( cbTCC) cells were used to determine effects of calcitriol and its related analogues on antioxidant enzyme gene expression, protein expression and activity. Catalase mRNA was increased in response to calcitriol (10−7 M), and seocalcitol (10−7 and 10−9 M). MnSOD mRNA was decreased in response to calcitriol at 10−7 M. Catalase was significantly increased in response to calcitriol (10−7 and 10−9 M), and seocalcitol (10−9 M). Catalase enzymatic activity increased in response to calcitriol, seocalcitol and analogue V (10−9 M). In addition, global gene expression analysis identified the involvement of mitogen-activated protein kinase ( MAPK) signalling in cbTCC's response to calcitriol and seocalcitol treatment. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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