| Abstrakt: |
Plants used in traditional medicine may constitute an important source of new biologically active compounds. There is a continuous and urgent need to discover new antimicrobial compounds with diverse chemical structures and novel mechanism of action for new and re-emerging infectious diseases. In the present study we carried out the antimicrobial effect and analysis of phytochemical constituents of different plant parts of Chrysanthemum morifolium Ramat. (Asteraceae). Pathogenically significant bacteria (Salmonella typhi-MTCC-733, Staphylococcus aureus- MTCC-7443, Pseudomonas aeruginosa-MTCC-7296, Mycobacterium tuberculosis-MTCC-300) and fungus (Microsporum canis-MTCC-2820, Epidermophyton floccossum-MTCC-613, Trichophyton rubrum -MTCC-296 and Aspergillus candidus -MTCC-1989) were selected for the study. The extract of root, stem, leaf and flower of plant were prepared in methanol and aqueous solutions in different doses (100 μl, 200 μl, 300 μl and 400 μl) were selected for study by agar disk diffusion method. We found significantly effective antibacterial property of Chrysanthemum morifolium at 400 μl concentration against Salmonella typhi, Staphylococcus aureus and Pseudomonas aeruginosa, but found completely ineffective against Mycobacterium tuberculosis only. The significantly effective antifungal activity was found also at 400 μl concentration against Microsporum canis, Epidermophyton floccossum and Trichophyton rubrum but found completely ineffective against Aspergillus candidus only. The secondary metabolites of extracts effective against microorganisms was evaluated and were determined as saponins, tannins, cardiac glycosides, alkaloids, flavonoids, steroids and terpenoids. The HPLC profiling was also performed and identified as Luteolin which is probably effective agent against above microorganisms. [ABSTRACT FROM AUTHOR] |
