| Autor: |
Kabasawa, Yuhji, Asahina, Izumi, Gunji, Akemi, Omura, Ken |
| Předmět: |
|
| Zdroj: |
DNA & Cell Biology; Sep2003, Vol. 22 Issue 9, p541, 6p |
| Abstrakt: |
Bone morphogenetic protein (BMP) induces bone formation in young rodents, but aging causes a reduction in the bone-forming ability of BMP. Most patients who require bone reconstruction are relatively old. Accordingly, we examined whether anabolic hormones could restore the bone inductive activity of rhBMP-2 in aged rats. rhBMP-2 in a carrier pellet was implanted subcutaneously in both 4- and 50-week-old female Wistar rats. PTH, PGE[sub 2], or 1,25(OH)[sub 2]D[sub 3] was injected every day during the period of BMP implantation. The pellets were harvested, and were examined both histologically and biochemically 2 weeks after implantation. Bone-forming ability was measured by alkaline phosphatase (ALP) activity and calcium (Ca) content. Pellets in 50-week-old rats showed a significant reduction in bone formation compared to pellets in 4-week-old rats. However, daily injections of PTH into 50-week-old rats restored both ALP activity (103 ± 4.6%) and Ca content (105 ± 2.6%). 1,25(OH)[sub 2]D[sub 3] and PGE[sub 2] also restored Ca content (103 ± 4.5% and 98 ± 3.8%, respectively) and stimulated ALP activity (142 ± 2.3% and 133 ± 3.6%). These results show that the administration of these hormones restores bone-forming ability in aged rats. A combination treatment of these hormones with rhBMP-2 might be applicable to the reconstruction of bone defects in elderly patients. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
