| Autor: |
Colton, Deanna M., Stoudenmire, Julie L., Stabb, Eric V. |
| Předmět: |
|
| Zdroj: |
Molecular Microbiology; Sep2015, Vol. 97 Issue 6, p1114-1127, 14p, 1 Diagram, 1 Chart, 8 Graphs |
| Abstrakt: |
Proteobacteria often co-ordinate responses to carbon sources using CRP and the second messenger cyclic 3′, 5′- AMP ( cAMP), which combine to control transcription of genes during growth on non-glucose substrates as part of the catabolite-repression response. Here we show that cAMP- CRP is active and important in V ibrio fischeri during colonization of its host squid E uprymna scolopes. Moreover, consistent with a classical role in catabolite repression, a cAMP- CRP-dependent reporter showed lower activity in cells grown in media amended with glucose rather than glycerol. Surprisingly though, intracellular cAMP levels were higher in glucose-grown cells. Mutant analyses were consistent with predictions that CyaA was responsible for cAMP generation, that the EIIAGlc component of glucose transport could enhance cAMP production and that the phophodiesterases CpdA and CpdP consumed intracellular and extracellular cAMP respectively. However, the observation of lower cAMP levels in glycerol-grown cells seemed best explained by changes in cAMP export, via an unknown mechanism. Our data also indicated that cAMP- CRP activity decreased during growth on glucose independently of crp's native transcriptional regulation or cAMP levels. We speculate that some unknown mechanism, perhaps carbon-source-dependent post-translational modulation of CRP, may help control cAMP- CRP activity in V . fischeri. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
Plný text