| Abstrakt: |
The growing of biomass, lipid synthesis and lipid fractions of streptomycetes isolated from soils of Republic of Moldova was studied. Strains of streptomycetes were cultivated on four media: complex media (M-I and R – main source of Carbon and Nitrogen – corn flour and starch) and synthetics classic media (Czapek and Dulaney). After cultivation for 5 days at 27°C on agitator, it was found that the amount of biomass after cultivation on a complex media, varies from 3.5 to 19.6 g/l, and less after cultivation on synthetic media – 2.26-6.32 g/l. The amount of lipids on the complex medium is from 1.5% to 18.69%, and on synthetic media – 3.12-12.6%.The qualitative composition of lipids in the studied strains of streptomycetes was almost identical: the main lipid fraction were submitted by phospholipids, sterols, mono-di and tri-glycerides, sterol esters and waxes. Strains S. ssp. 12, 19 and 36, the amount of phospholipids was more after cultivation on synthetic media (12.6-15.4%) and at S. ssp. 47, 66 and 205 - on complex media (11.1-17.16%), the amount sterols changed, depending on the strain and nutritive medium from 7.3 to 14.6%, triglycerides from 16.48 to 55.1%, sterols esters – 10.8-20.0%, waxes – 5.1-36.5%, free fatty acids from 8.11 to 18.8 %. Of greatest interest are strains S. ssp. 19, 36, 47 and 66 constituting the largest amount of biomass after cultivation on complex media (12.1-19.6%), wherein the amount of lipids is from 5.9 to 18.7% and the physiologically active lipid fraction (phospholipids, sterols) - 10.75-17.2% and 9.8-14.6%, respectively. Indicators allow us to consider the strains as the basis of new biological drug for animal husbandry. [ABSTRACT FROM AUTHOR] |
