| Autor: |
Akhter, Shamim, Rakha, Bushra Allah, Ansari, Muhammad Sajjad, Hassan Andrabi, Syed Murtaza, Ullah, Nemat |
| Zdroj: |
Pakistan Journal of Zoology; Apr2011, Vol. 43 Issue 2, p273-277, 5p |
| Abstrakt: |
We investigated the effect of ascorbic acid and α-tocopherol supplementation in skim milk extender on the preservability of buffalo bull spermatozoa stored at 5°C. For this purpose, semen samples were collected from Nili-Ravi buffalo (Bubalus bubalis) bulls (n = 3) and diluted at 37°C with skim milk extender containing ascorbic acid (0.5mM) or α-tocopherol (1.0mM) or without any supplement (Control). The sperm concentration in the extender was adjusted at 10 x 106 motile spermatozoa per ml. Diluted semen was cooled from 37°C to 5°C in 2 hours and stored at 5°C for five days. Semen quality assays for sperm motility, plasma membrane integrity, normal apical ridge and abnormalities were performed at 1st, 3rd and 5th day of storage. Percentage of sperm motility was lower (P < 0.05) in extender containing α-tocopherol as compared to ascorbic acid and control at 1st and 3rd day of storage. However, it did not differ at 5th day of storage. There was no difference (P > 0.05) in percentage of plasma membrane integrity and normal apical ridge of buffalo bull spermatozoa for five days of storage. Moreover, sperm abnormalities (head, mid piece and tail) remained similar (P > 0.05) in all experimental extenders for five days to storage. It is concluded that ascorbic acid and α-tocopherol addition in skim milk did not improve the semen quality of Nili-Ravi buffalo bull spermatozoa stored at 5°C for five days. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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