Cinnamaldehyde Contributes to Insulin Sensitivity by Activating PPARδ, PPARγ, and RXR.
| Autor: | Li, Juan-E, Futawaka, Kumi, Yamamoto, Hiroyuki, Kasahara, Masato, Tagami, Tetsuya, Liu, Tong-Hua, Moriyama, Kenji |
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| Předmět: |
RNA analysis
ALDEHYDES ANIMAL experimentation BIOLOGICAL assay CELL culture CELL receptors CINNAMON FAT cells INSULIN resistance MICE POLYMERASE chain reaction RESEARCH funding RETINOIDS STATISTICS PLANT extracts DATA analysis REVERSE transcriptase polymerase chain reaction DATA analysis software PIOGLITAZONE PEROXISOME proliferator-activated receptors DESCRIPTIVE statistics ONE-way analysis of variance |
| Zdroj: | American Journal of Chinese Medicine; 2015, Vol. 43 Issue 5, p879-892, 14p, 6 Graphs |
| Abstrakt: | Cinnamon is a traditional folk herb used in Asia and has been reported to have antidiabetic effects. Our previous study showed that cinnamaldehyde (CA), a major effective compound in cinnamon, exhibited hypoglycemic and hypolipidemic effects together in db/db mice. The aim of the present study was to elucidate the molecular mechanisms of the effects of CA on the transcriptional activities of three peroxisome proliferator-activated receptors, (PPAR) α, δ, and γ. We studied the effects of CA through a transient expression assay with TSA201 cells, derivatives of human embryonic kidney cell line (HEK293). Quantitative reverse transcription polymerase chain reaction (qRT-PCR) analysis was also performed to evaluate mRNA expression levels. We show here that CA induced PPARδ, PPARγ and retinoid X receptor (RXR) activation. CA may activate PPARγ in a different manner than pioglitazone, as CA selectively stimulated PPARγ S342A mutant while pioglitazone did not. In addition, CA and L-165041 had a synergistic effect on PPARδ activation. To gather the biological evidence that CA increases PPARs transcription, we further measured the expressions of PPARδ and PPARγ target genes in 3T3-L1 adipocytes. The data showed CA induced the expression of PPARδ and PPARγ target genes, namely aP2 and CD36, in differentiated adipocytes. As a result, PPARδ, PPARγ and their heterodimeric partner RXR appear to play a part in the CA action in the target tissues, thereby enhancing insulin sensitivity and fatty acid β-oxidation and energy uncoupling in skeletal muscle and adipose tissue. [ABSTRACT FROM AUTHOR] |
| Databáze: | Complementary Index |
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