| Autor: |
Child, Hannah Winifred, Hernandez, Yulán, Conde, João, Mullin, Margaret, Baptista, Pedro, de la Fuente, Jesus Maria, Berry, Catherine Cecilia |
| Zdroj: |
Nanomedicine; 2015, Vol. 10 Issue 16, p2513-2525, 13p |
| Abstrakt: |
Aims: RNAi is a powerful tool for gene silencing that can be used to reduce undesirable overexpression of oncogenes as a novel form of cancer treatment. However, when using RNAi as a therapeutic tool there is potential for associated gene effects. This study aimed to utilize gold nanoparticles to deliver siRNA into HeLa cells. Results: Knockdown of the c-myc oncogene by RNAi, at the RNA, protein and cell proliferation level was achieved, while also identifying associated gene responses. Discussion: The gold nanoparticles used in this study present an excellent delivery platform for siRNA, but do note associated gene changes. Conclusion: The study highlights the need to more widely assess the cell physiological response to RNAi treatment, rather than focus on the immediate RNA levels. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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