| Autor: |
Ritchie, H. H., Li, X. R., Hanks, C. T., Knudtson, K., Wang, L.-H. |
| Předmět: |
|
| Zdroj: |
Connective Tissue Research; Apr2002, Vol. 43 Issue 2/3, p331-337, 7p |
| Abstrakt: |
Two highly expressed noncollagenous proteins associated with dentin mineralization, dentin sialoprotein (DSP) and phosphophoryn (PP), are encoded by a single DSP-PP transcript. To better understand how DSP-PP transcripts are regulated, we have determined the DSP-PP transcription start site, sequenced its 5′ flanking region, and analyzed the transcriptional activity of the gene promoter out to -1615 bp. Comparison of the rat cDNA sequence with the mouse, rat and human genes clearly indicates high sequence conservation within the DSP-PP 5′ flanking region, implicating the possible presence of highly conserved gene regulatory cis elements. Among a number of conserved transcription sites identified in the 5′ flanking region, we demonstrate that the conserved Y box sequence (ATTGG) can specifically bind nuclear extracts from mouse MDPC23 cells. This sequence (located within the -57 bp/-52 bp 5′ flanking region) therefore likely represents one DSP-PP transcriptional regulatory sequence. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
