| Autor: |
Kashirina, E., Reshetov, P., Alekseeva, L., Khlgatyan, S., Ryazantsev, D., Zubov, V., Guryanova, S., Svirshchevskaya, E. |
| Zdroj: |
Nanotechnologies in Russia; Jul2015, Vol. 10 Issue 7/8, p627-635, 9p |
| Abstrakt: |
Allergen preparations intended for the specific therapy of IgE mediated allergy must not induce side effects due to IgE binding. To prevent IgE binding, recombinant Der f 1 and Der f 2, major allergens from house dust mites (HDMs) Dermatophagoides farinae, are capsulated into nanoparticles developed from biodegradable polymers. Nanoparticles 80 nm in diameter and with zeta potential +8 mV are generated from amphiphilic chitosan derivative N-lauryl- N'-succinoylchitosan (LSC). Der f 1 and Der f 2 proteins are introduced during nanoparticle formation. To cover allergens additionally, nanoparticles are coated by an alginate shell. As a result of polyelectrolyte interaction between positively charged LSC and negatively charged alginic acid, constructions of the 'core-shell' type are formed which are 90-140 nm in diameter and zeta potential is-13 to-20 mV. We demonstrate that the capsulation of allergens into LSC nanoparticles significantly decreases IgE reactivity while core-shell construction masks the allergens completely, as was shown using IgE positive sera from patients allergic to HDMs. We conclude that constructions of a core-shell type with allergens incorporated in the core particles could increase the safety of preparations for the specific immunotherapy of allergies. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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