| Autor: |
Niessen, Anna, Heyder, Petra, Krienke, Stefan, Blank, Norbert, Tykocinski, Lars-Oliver, Lorenz, Hanns-Martin, Schiller, Martin |
| Předmět: |
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| Zdroj: |
Journal of Cell Science; Jul2015, Vol. 128 Issue 14, p2443-2453, 11p, 7 Graphs |
| Abstrakt: |
A dysregulation in the clearance of apoptotic material is considered a major pathogenetic factor for the emergence of autoimmune diseases. Apoptotic-cell-derived membrane microparticles (AdMPs), which are released from the cell surface during apoptosis, have been implicated in the pathogenesis of autoimmunity. Also of importance are cytokines, such as interferon-α (IFN-α), which is known to be a major player in patients with systemic lupus erythematosus (SLE). This study investigates the combined effect of AdMPs and IFN-α on professional phagocytes. In the presence of IFN-α, phagocytosis of AdMPs by human monocytes was significantly increased in a dose- dependent manner. The combination of AdMPs and raised IFN-α concentrations resulted in an increase in the secretion of pro- inflammatory cytokines and an upregulation of surface molecule expression involved in antigen uptake. In addition, macrophage polarisation was shifted towards a more inflammatory type of cell. The synergism between IFN-α and AdMPs seemed to be mediated by an upregulation of phosphorylated STAT1. Our results indicate that IFN-α, together with AdMPs, amplify the initiation and maintenance of inflammation. This mechanism might especially play a crucial role in disorders with a defective clearance of apoptotic material. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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