| Autor: |
Sung, HH, Castro, I, González, S, Aguilera, S, Smorodinsky, NI, Quest, AFG, Bahamondes, V, Alliende, C, Cortés, J, Molina, C, Urzúa, U, Barrera, M‐J, Hermoso, M, Herrera, L, Leyton, C, González, M‐J |
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| Zdroj: |
Oral Diseases; Sep2015, Vol. 21 Issue 6, p730-738, 9p, 1 Diagram, 1 Chart, 4 Graphs |
| Abstrakt: |
Objectives To evaluate the expression and localization of MUC1/ SEC and MUC1/Y isoforms in labial salivary glands ( LSG) from Sjögren's syndrome patients ( SS patients), as well as their in vitro expression induced by cytokines. Subjects and methods Labial salivary gland from 27 primary SS patients and 22 non-SS sicca subjects were studied. Relative MUC1/SEC and MUC1/Y m RNA levels were determined by q PCR and protein levels by Western blotting. Induction of mucin mRNAs was assayed in vitro. Immunohistochemistry was used for localization. Results Relative MUC1/SEC and MUC1/Y mRNA and protein levels were significantly higher in LSG from SS patients. These mRNAs were induced by cytokines. MUC1/SEC and MUC1/Y were detected in acini apical region of control LSGs, and significant cytoplasmic accumulation was observed in acini of SS patients. MUC1/Y localized in acinar nuclei and cytoplasm of inflammatory cells of LSG from SS patients. A strong positive correlation was observed between cellular MUC1/SEC levels and glandular function determined by scintigraphy. Conclusions We show for the first time that MUC1/ SEC and MUC1/Y are expressed in LSG of both SS patients and non- SS sicca subjects. The observed overexpression and aberrant localization of MUC1/ SEC and MUC1/Y and their induction by pro-inflammatory cytokines may favor the perpetuation of the inflammatory environment that disrupts the salivary glandular homeostasis in SS patients. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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