| Autor: |
Zahran, F, Ghalwash, D, Shaker, O, Al‐Johani, K, Scully, C |
| Předmět: |
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| Zdroj: |
Oral Diseases; Sep2015, Vol. 21 Issue 6, p739-747, 9p, 4 Charts, 4 Graphs |
| Abstrakt: |
Objective This study investigated the use of three salivary micro RNAs (mi RNA-21, mi RNA-184, and mi RNA-145) as possible markers for malignant transformation in oral mucosal lesions. Materials and methods Salivary whole unstimulated samples were collected from a study group of 100 subjects, consisting of 20 clinically healthy controls, 40 patients with oral potentially malignant disorders ( PMDs) [20 with dysplastic lesions and 20 without dysplasia], 20 with biopsy-confirmed oral squamous cell carcinoma ( OSCC), and 20 with recurrent aphthous stomatitis ( RAS) as disease controls. Total RNA was isolated and purified from saliva samples using the micro RNA Isolation Kit ( Qiagen, UL). mi RNA expression analysis was performed using qRT- PCR ( Applied Biosystems). Results There was a highly significant increase in salivary mi RNA-21 and mi RNA-184 in OSCC and PMD (with and without dysplasia) when compared to healthy and disease controls ( P < 0.001). Conversely, mi RNA-145 levels showed a highly significant decrease in OSCC and PMD overall ( P < 0.001). RAS cases showed no significant difference from normal controls in any measured mi RNA ( P > 0.05). The only micro RNA to discriminate between OSCC and PMD with dysplasia was mi RNA-184. When receiver operating characteristic curves were designed for the three mi RNAs, cutoff points delineating the occurrence of malignant change were a fourfold increase in mi RNA-21 with specificity 65% and sensitivity 65%, a 0.6 decrease in mi RNA-145, with specificity 70% and sensitivity 60%, and a threefold increase of mi RNA-184, with specificity 75% and sensitivity 80%. Calculating the area under the curve revealed that mi RNA-184 was the only one among the studied mi RNAs that provided good diagnostic value. Conclusion Salivary determination of the mi RNAs tested might furnish a noninvasive, rapid adjunctive aid for revealing malignant transformation in oral mucosal lesions, particularly mi RNA-184. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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