| Autor: |
Xu, Yang, Xu, Ling, Xia, Yuan, Guan, Chao-Jian, Guo, Qing-Xiang, Fu, Yao, Wang, Chen, Li, Yi-Ming |
| Předmět: |
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| Zdroj: |
Chemical Communications; 8/28/2015, Vol. 51 Issue 67, p13189-13192, 4p |
| Abstrakt: |
Rapid and catalyst-free hydrazone ligation reaction between ortho-halobenzaldehyde derivatives and peptide/protein hydrazides was observed at neutral pH and room temperature. 2-Chlorobenzaldehyde exhibited the fastest reaction and highest conversion rates among the series of ortho-halobenzaldehydes. The resulting hydrazone-containing bioconjugation products were also found to be fairly stable under experimental conditions. The new ligation strategy was successfully used for protein C-terminal labeling and should provide a practical approach for the modification of proteins. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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