| Autor: |
Xue-Feng Tan, Jian-Bing Qin, Guo-Hua Jin, Mei-Ling Tian, Hao-Ming Li, Hui-Xia Zhu, Xin-Hua Zhang, Jin-Hong Shi, Zhen Huang |
| Předmět: |
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| Zdroj: |
Cell Biology International; Sep2010, Vol. 34 Issue 9, p877-882, 6p |
| Abstrakt: |
NSCs (neural stem cells) provide a powerful research tool for the design and discovery of new approaches to cell replacement therapy during brain repair. However, the usefulness of this tool has been particulaly obstructed by limited neuronal differentiation of NSCs. Brn-4, a member of the POU domain family of transcription factors, has been previously implicated in the development of neurons by expression analysis. Here, we directly investigated the effects of Brn-4 on the neuronal differentiation and development of NSCs derived from the E13 rat midbrain. We found that Brn-4 knockdown in NSCs resulted in a significant decrease of MAP-2-positive neurons with immature morphology. Overexpression of Brn-4 in NSCs markedly increased the production and maturation of newborn neurons. These results suggest that Brn-4 has a critical role in the neuronal differentiation of mesencephalic NSCs and the maturation of newborn neurons. Brn-4 may be utilized to manipulate NSCs for gene and cell therapy of several neurological diseases. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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