| Autor: |
Bassoli, Bruna Kempfer, Cassolla, Priscila, Borba‐Murad, Glaucia Regina, Constantin, Jorgete, Salgueiro‐Pagadigorria, Clairce Luzia, Bazotte, Roberto Barbosa, Souza, Helenir Medri |
| Zdroj: |
Cell Biochemistry & Function; Jun2015, Vol. 33 Issue 4, p183-187, 5p |
| Abstrakt: |
Coffee is the main source of chlorogenic acid in the human diet, and it contains several chlorogenic acid isomers, of which the 5-caffeoylquinic acid (5-CQA) is the predominant isomer. Because there are no available data about the action of chlorogenic acids from instant coffee on hepatic glucose-6-phosphatase (G-6-Pase) activity and blood glucose levels, these effects were investigated in rats. The changes on G-6-Pase activity and liver glucose output induced by 5-CQA were also investigated. Instant coffee extract with high chlorogenic acids content (37.8%) inhibited (p < 0.05) the G-6-Pase activity of the hepatocyte microsomal fraction in a dose-dependent way (up to 53), but IV administration of this extract did not change the glycaemia (p > 0.05). Similarly, 5-CQA (1 mM) reduced (p < 0.05) the activity of microsomal G-6-Pase by about 40%, but had no effect (p > 0.05) on glucose output arising from glycogenolysis in liver perfusion. It was concluded that instant coffee extract with high content of chlorogenic acids inhibited hepatic G-6-Pase in vitro, but failed to reduce the glycaemia probably because the coffee chlorogenic acids did not reach enough levels within the hepatocytes to inhibit the G-6-Pase and reduce the liver glucose output. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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